Download Adipose-Derived Stem Cells: Methods and Protocols by Christine Gagliardi, Bruce A. Bunnell (auth.), Jeffrey M. PDF

By Christine Gagliardi, Bruce A. Bunnell (auth.), Jeffrey M. Gimble, Bruce A. Bunnell (eds.)

During the earlier decade, a variety of medical disciplines have followed using adipose-derived stem/stromal cells (ASCs) as an immense software for study and discovery. In Adipose-Derived Stem Cells: equipment and Protocols, specialists from the sector, together with contributors of the esteemed overseas Federation of Adipose Therapeutics and technology (IFATS), offer outlined and tested protocols in an effort to additional codify the usage of those strong and available cells. With chapters prepared round ways spanning the invention, pre-clinical, and medical strategies, a lot of the emphasis is put on human ASC, whereas extra concepts regarding small and massive animal species are incorporated. As a quantity within the hugely profitable equipment in Molecular Biology™ sequence, the targeted contributions comprise introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, comfortably reproducible laboratory protocols, and notes on troubleshooting and keeping off recognized pitfalls. complete and state-of-the-art, Adipose-Derived Stem Cells: equipment and Protocols serves as an essential reference textual content for skilled researchers in addition to new scholars at the route to extra exploring the really good capability of ASCs.

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Complete stromal medium: DMEM-Ham’s F12 supplemented with 1% antibiotic/antimycotic solution and 10% FBS. 3. Complete stromal medium. T25 tissue culture flasks. Trypsin–EDTA: store long term at −20°C. Cryopreservation medium: 80% FBS, 10% DMEM-Ham’s F-12, 10% dimethylsulfoxide (DMSO). “Mr. Frosty” freezing container (alcohol container). Cryovials. 4. PBS. Trypsin–EDTA. 4% Trypan Blue. Hemocytometer. Complete stromal medium. 12-well culture plates. 5. DMEM-Ham’s F-12 medium. Characterized FBS (10%).

Spencer Abstract Mesenchymal stromal cell research has advanced significantly over the last decade, especially in the area of adult adipose-derived stem cells (ASCs). There are significant species differences in ASC harvest, as well as in vitro doubling time and differentiation. Selection of appropriate animal models for preclinical evaluations is critical for optimization and validation of ASC therapeutic effects. As such, in vitro studies are necessary precursors to in vivo ASC applications. Additionally, to elucidate environmental, disease, or trauma effects on native stromal cell populations, characterization of cells from unexposed individuals is necessary.

Adipose-Derived Stem Cells: Methods and Protocols, Methods in Molecular Biology, vol. 1007/978-1-61737-960-4_2, © Springer Science+Business Media, LLC 2011 17 18 Yu et al. 2. 1. 2. Supplies Subcutaneous adipose tissue samples obtained from liposuction aspirates (see Note 1). 200-ml plastic centrifugation bottles. 2-mm filter units. 50-ml conical tubes. 2-ml tubes. Scissors. Hemocytometer. Freezing apparatus (alcohol container). Fluorochrome-conjugated monoclonal antibodies against stromal, hematopoietic, endothelial, pericytic, and related cell surface antigens.

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